Lab Bioreactor

Laboratory scale bioreactor are generally used in microbiology laboratories of scientific research institutes and enterprises, and are ideal tools for precision fermentation experiments. It can be applied to the screening of medium formulations for microbial fermentation culture, the optimization of fermentation process parameters, and the verification of production processes and strains. Especially the crude mash medium can be adapted.

The laboratory scale bioreactor is uniquely configured, flexible, easy to operate, and occupies a very small area. The laboratory scale bioreactor adopts a full computer touch screen control system, color touch screen display, and has a powerful expansion function, supporting up to 24 fermentation parameters control, It is an experimental platform for research on fermentation process conditions and precision cultivation.

Classification of Laboratory scale bioreactor

Laboratory scale bioreactor are divided into mechanical stirring and ventilation Laboratory scale bioreactor and non-mechanical stirring and ventilation Laboratory scale bioreactor. According to the growth and metabolism needs of microorganisms, they are divided into aerobic Laboratory scale bioreactor and anaerobic Laboratory scale bioreactor.

The main components of the laboratory scale bioreactor:

1. Tank: It is mainly used to cultivate and ferment various bacterial cells, and the sealing is better;

2. Stirring paddle: There is a stirring paddle in the tank, which is used for continuous stirring during the fermentation process;

3. The Sparger ventilated at the bottom is used to introduce the air or oxygen required for the growth of the bacteria;

4. Sensor: There are control sensors on the top plate of the tank, commonly used are pH electrodes and DO electrodes, which are used to monitor the changes of pH and DO of the fermentation broth during the fermentation process;

5. Controller: used to display and control fermentation conditions, etc.

The main body of the laboratory scale bioreactor tank is provided with interlayer and thermal insulation layer, which can be heated, cooled and thermally insulated. The tank body and the upper and lower elliptical heads are processed by spinning R angle. The inner wall of the tank is mirror polished, which is hygienic and has no dead ends. The fully enclosed design ensures that the materials are always mixed and fermented in a pollution-free state. The equipment is equipped with air breathing holes, CIP cleaning nozzles, manholes, inlet and outlet and other devices.

The use method and operation steps of the laboratory scale bioreactor:

1. Calibrate pH electrode and dissolved oxygen electrode;

2. Sterilize the tank. Put the culture medium into the tank as required, seal the tank as required, and put the tank into a large sterilizer for sterilization (115℃, 30 minutes);

3. After the tank is cooled, place it on the fermentation stand and install it well; turn on the cooling water, turn on the power of the air pump, connect the ventilation pipe to start ventilation, adjust the air intake knob to make the ventilation volume appropriate; turn on the power of the fermentation tank, set the temperature, pH, stirring speed, etc., start the machine and rotate for 30min at 640r/min, and set the dissolved oxygen electrode to 100;

4. After the temperature is stable and the parameters are correct, connect the pre-prepared inoculum to start the fermentation timing and start recording various parameters;

5. After the fermentation is completed, clean the tank body and the electrode, and insert the electrode into the triangular flask for use;

Daily maintenance and maintenance methods of laboratory scale bioreactor tanks:

1. The laboratory scale bioreactor tank should be operated and used strictly according to the working pressure and working temperature calibrated on the product nameplate, so as to avoid damage to the stainless steel tank and personal danger.

2. The laboratory scale bioreactor tank strictly abides by the regulations on cooling and oil injection in the product instruction manual, and does a good job in the maintenance and maintenance of the mixing tank.

3. When all valves of the laboratory scale bioreactor are used, the valve stem (needle) should be slowly rotated to press the cover tightly to achieve the sealing effect. Do not use excessive force when closing, so as not to damage the sealing surface.

4. The electrical control instruments of the laboratory scale bioreactor tank should be operated by special personnel, and overload protection facilities should be set up as required.

Cleaning process of laboratory scale bioreactor:

1. After the laboratory scale bioreactor tank is used, rinse it with clean water for 30 minutes, and the discharged water is clear and free of residues.

2. Use sodium hydroxide solution with a temperature of 80-85°C and a concentration of 4% to perform CIP cycle for 30min.

3. Rinse the fermentation tank with water for 20min.

4. Use chlorine dioxide solution with concentration of 200ppm or formaldehyde solution with concentration of 2% to carry out CIP cycle for 30-40min.

5. Rinse the fermentation tank with sterile water for 20-30 minutes.

6. Before entering the tank, pump hot water at 85-90°C from the cone bottom of the bioreactor until the water is discharged from the exhaust pipe. After soaking for 30-40min, drain it and cool it down to 8-9°C for use. At the same time, in order to ensure the good hygienic effect of the fermentation tank, it should be entered into the fermentation tank from the manhole every two months, and the dead corners and residues on the top should be thoroughly cleaned.

Cleaning is to remove as much dirt as possible from the surfaces of tanks, pipes and equipment. If the cleaning and sterilization are not thorough, the residual fouling on the inner wall of the equipment and the reproduction of microorganisms will weaken the effect of the bactericide. Due to the action of static electricity and other factors, these dirts are deposited on the surface of the inner wall of the equipment, and provide a habitat for the growth of microorganisms. Microbial colonies must be removed during washing. Because the bactericide is suitable for surface sterilization, it has little effect on the bacteria living inside, and it is easy to cause recontamination.

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